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Это обсуждение публикации https://olympiads.bc-pf.org/biology/national/2022/10
Это обсуждение публикации https://olympiads.bc-pf.org/biology/national/2022/10
Первый вопрос, суть этой задачи в том, чтобы просто обработать информацию, или есть посвященная этому тема? Если да, буду благодарна если прикрепите материалы или ссылки на неё.
Второй вопрос, какая связь у процесса транскпрции с результатами электрофореза - как ответить на 39 и 41 вопрос.
Почему в 38 вопросе ответ Н, если там вовсе нет никакой отметки?
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It is my first time here so I am sorry for my ignorance If I will do something wrong here. Before doing this q, learning protein-protein interaction assays won’t hurt you (something like LCI, COIP, GST pulldown tbh reading wiki page is enough, reading papers might be redundant but you may aslo try them). Next, I am sure you can answer 3/4 of the q by just looking at the blot (figure 2), you see no band in 32P + ester treatment, which implies that your RB is not phosphorylated but it does not necessarily mean you do not have any RB at all (look at the 32S + ester treatment, there is still a band = you are running your protein aliqout) hence 38 is F. The same logic is appliable to 39 (no 32P = more bound to E2F) 39 is T. Then, since we know after ester treatment there are more bound fraction of RB, we can conclude that there are no S genes transcription = no proliferation (40 is T). Now, for the last one we shpuld look carefully at COIP results (figure 3). We are using antI-E7 sepharose beads and then running western on our precipitate. Let’s look at the 6th example, we see clear band here, which means we actually detected RB in E7 precipitate, so that there actually interaction between RB and E7. But if we eyeball the 3rd example, there are no band at all? Rn I might sound quite stupid, but I interpreted this result as E7 competing with E2F for RB. During ether treatment, when there are no bound fraction of E2F with RB, E7 binds with RB (at the sime site as E2F) making E2F unavailable to rebind with RB, promoting cell proliferation. 41 is T (FTTT the same as in ans key)
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